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Catalog# Product Description |
ICP0506 Affinity purified rabbit polyclonal anti-methylated lysine antibody is developed using a methylated protein as the immunogen. The methyllysine specific antibodies are affinity purified with N-e-mono-methyllysine and di-methyllysine on agarose as the affinity matrix. The purified antibody was conjugated to fluorescein. The conjugated antibody was tested to localize methylated proteins. |
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Species
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Rabbit
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Formulation
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250 μg/mL in PBS, 50% glycerol
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Immunogen
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Methylated KLH conjugates
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Purification
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N-e-mono-methyllysine and di-methyllysine on agarose
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Specificity
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Recognizes proteins methylated on lysine residues (mono and di-methyllysine). It does not cross react with acetylated proteins. The cross reactivity to tri-methyllysine has not been tested.
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Conjugation
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With fluorescein isothiocyanate (FITC)
FITC/Ab molar ratio - 10:1
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Applications
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IF (1:50 - 1:100)
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Storage & Stability
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Product is stable for several weeks at 4°C. For extended storage, store product at –20oC. Expiration date is six months from date of shipping if properly stored.
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