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Catalog# Product Description |
ICP0205 Affinity purified rabbit polyclonal anti-p42 MAPK antibodies are developed with the C-terminal peptide of human MAPK (ERK2). |
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Western blot analysis of mouse brain and spleen samples immunoprecipitated with anti-ERK2 antibody: left two lanes with ERK 2 and right two lanes with pre-immune serum.
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Species
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Rabbit
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Formulation
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250 μg/mL in TBS, 50% glycerol, 0.01% NaN3
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Immunogen
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Human p42 ERK2 C-terminal peptide-KLH conjugates
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Purification
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The antibody is affinity purified with the p42 ERK2 C-terminal peptide on agarose.
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Specificity
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The antibody recognizes human, rat, bovine, mouse and rabbit p42 ERK2. It does not cross-react with p44 ERK1.
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Applications
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ELISA; WB; IP
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Scientific Description
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Maturation of mitogen-activated protein kinase (MAPK), also known as extracellular signal regulated kinase 2 (ERK2), is involved in the eukaryotic signalling process. [1] It is activated by a dual phosphorylation on threonine and tyrosine residues by the dual-specificity kinase MKK1/2. After stimulation, ERK2 p42 translocates to the nucleus. [2] Once phosphorylated, ERK2 can form dimers with phosphorylated and unphosphorylated ERK2 partners. ERK2 can also phosphorylate many substrates, for instance EGFR, c-PLA2, Elk1/TCF and c-Myc. [3-5]
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Storage & Stability
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Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly.
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Product Specific References |
1. Mol. Cell Biol. 1992. 12: 915–927. doi:10.1128/MCB.12.3.915 2. J. Biol. Chem. 1991. 266(23): 15266–15276. 3. Cell. 1993. 72: 269–278. doi:10.1016/0092-864(93)90666-E 4. J. Biol. Chem. 1992. 267(34): 24796-24804. |
