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Catalog # Product Description |
ICP0903 This high affinity anti-Flg (DYKDDDDK) antibody is This high affinity anti-Flg (DYKDDDDK) antibody is developed using the Flg peptide (DYKDDDDK) conjugated to KLH and affinity-purified with the DYKDDDDK peptide. The purified antibody was conjugated to FITC. The antibody is useful for detection, isolation and localization of DYKDDDDK-tag proteins |
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Western blot analysis of the cell extract with Flg-tag protein expression (lane 7 = 25 μg; lane 8 = 5 μg; lane 9 = 10 μg cell lysate)
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Species
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Rabbit
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Formulation
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250 μg/mL in PBS, 50% glycerol
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Immunogen
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Flg peptide (DYKDDDDK) KLH conjugates
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Conjugation
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Acylation with FITC; FITC/Ab ratio - 20:1
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Specificity
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The antibody recognizes DYKDDDDK-tag proteins.
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Applications
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IF (5 μg/sample)
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Scientific Description
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DYKDDDDK peptide (Flag-tag) is frequently used as an affinity tag to facilitate the detection, isolation, and localization of the DYKDDDDK-fusion proteins.
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Storage & Stability
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Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is six months year from date of shipping if stored properly.
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