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Catalog # Product Description |
CN1005 This antibody was developed using glutathione (GSH)-KLH conjugates and affinity purified with antigen-specific affinity chromatography. The antibody could be utilized for detection and quantization of reduced (GSH) and oxidized (GSSG) glutathione as well as glutathionylated proteins and peptides. |
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Western blot analysis of the glutathionylated proteins in MMRU cell lysates. Right lane: sample boiled with DTT.
IF staining of the MMRU cells (5 μg/mL).
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Species
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Rabbit
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Formulation
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250 μg/mL in PBS with 50% glycerol
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Immunogen
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Glutathione-KLH conjugates linked through -S- bonds
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Purification
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Antigen-specific affinity chromatography
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Specificity
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The pan-specific antibodies recognize both GSH and GSSG as well as glutathionylated proteins and peptides.
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Applications
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Quantitative ELISA; WB; IP (affinity chromatography); IF; IHC
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Scientific Description
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Glutathione is a tripeptide. It contains an unusual peptide linkage between the amine group of cysteine and the carboxyl group of the glutamate side chain. Glutathione, an antioxidant, protects cells from toxins such as free radicals. Glutathione can also modify proteins via disulfide bonds. Redox proteomics of glutathionylated proteins in oxidatively stressed human T lymphocytes have been reported.
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Storage & Stability
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Product is stable for several weeks at 4°C. For extended storage, store product at –20oC. Expiration date is three years from date of shipping if properly stored.
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Product Specific References |
1. PNAS. 2005. 102(39): 13998-14003. doi:10.1073/pnas.0504398102 2. J. Biol. Chem. 2007. 282(45): 32640-54. doi:10.1074/jbc.M702953200 |
